Remarkable recent experiments have demonstrated that single-celled organisms are able to respond not only to experienced changes in the past, but also to expected changes that have yet to occur – evidently exploiting temporal correlations in the environment to predict the future. But how reliably can a cell make such predictions, which are the mechanisms that implement them, and what are their fitness benefits?
In this project, you will address these questions experimentally, using single-cell microscopy to precisely quantify (in bits) the amount of predictive information cells extract from past experiences. The primary experimental vehicle will be a single-cell FRET method developed in our group (Keegstra et al., 2017 eLife; Kamino et al., 2020, Science Advances). Your measurements will quantify information transmission and prediction in the E. coli chemotaxis system – a cell signaling network for which sufficient quantitative knowledge exists to address further questions about mechanistic origins and functional benefits of prediction. You will collaborate closely with the theory group of Pieter Rein ten Wolde (AMOLF), using concepts from information theory and stochastic thermodynamics to design and interpret experiments.
For further information on the project, contact Tom Shimizu (email@example.com).
For more information about research in the Shimizu Group, see https://amolf.nl/research-groups/systems-biology.
You will need to meet the requirements for an MSc-degree, to ensure eligibility for a Dutch PhD examination.
We seek candidates with a strong background in experimental physics or quantitative biology and an interest in the biophysics of cellular information processing. Prior experience with microscopy is preferred.
The position is intended as full-time (40 hours / week, 12 months / year) appointment in the service of the Netherlands Foundation of Scientific Research Institutes (NWO-I) for the duration of four years, with a starting salary of € 2407 and a range of employment benefits. Ideally with a starting date before June 2021. After successful completion of the PhD research a PhD degree will be granted at University. Several courses are offered, specially developed for PhD-students. AMOLF assists any new foreign PhD-student with housing and visa applications and compensates their transport costs and furnishing expenses.
Prof.dr. Tom Shimizu
Group leader (Systems Biology)
Phone: +31 (0)20-754 7100
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|Title||PhD-student: Measuring predictive information in living cells|
|Job location||Science Park 104, 1098 XG Amsterdam|
|Published||December 2, 2020|
|Job types||PhD  |
|Fields||Experimental Physics,   Biophysics,   Cell Biology  |